ABSTRACT
Objective To study the function of LSD1 in the development of neurons and the influence of LSD1 on mood and memory-related behavior in mice. Methods The LSD1(flox / flox) transgenic mice were crossed with Nestin?cre(Tg) transgenic mice, using Cre?LoxP recombination system, to generate LSD1 conditional knockout of neural stem cell ( LSD1?CKO) mice, LSD1(flox/ flox) Nestin?cre(Tg) mice, and LSD1(flox/ flox) mice as control. The neuron proliferation in LSD1?CKO mice was further detected by immunofluorescence staining. At the same time, the mood and memory?related behavior of LSD1?CKO mice were examined using several methods:sucrose preference test ( SPT) , forced swimming test ( FST) and novel?object recognition ( NOR) assay. Results In the LSD1 brain?specific CKO mice, the neuron proliferation rate in the hippocampus was significantly reduced ( P=0. 023 ) , the preference for sucrose was reduced ( P =0. 0075 ) , immobility duration during the forced swimming test was increased (P<0. 05), and LSD1?CKO mice also exhibits memory?decline (P=0. 0019) during the novel?object recognition test. Conclusions Depletion of LSD1 in mouse brain neural stem cells leads to significant reduction of the neuron proliferation in the hippocampus. LSD1?CKO mice show more negative emotions and memory impairment.
ABSTRACT
Objective To examine the reliability and validity of the Rating Scale of the Reliability in Mental Disorder Medical History (RSRMDMH) in order to develop a reliable measure to determine the reliability in mental disorder medical history.Methods RSRMDMH was conducted by concept,clinical feature in mental disorder medical history and assessment scales for malingering and compensationneurosis.Final 14 items was decided after initial usage and modification.It was administered to 600 subjects including the cases involved forensic problems,patients in general psychiatric clinic.Results ①The Cronbach' s α coefficients of RSRMDMH was 0.901.The split-half reliability was 0.803-0.924.The test-retest reliability and scorer reliability were more than 0.588 (P＜0.01).②The cut score of assessing different degree of the reliability in mental disorder medical history separately was ≤ 11,12-22,≥23.③Based on the experts diagnosis,the accuracy rate of RSRMDMH for evaluating different degree of the reliability in mental disorder medical history were more than 86.1％,and total accuracy rate was 94.8％.④Correlation analysis showed significantly correlation between RSRMDMH and assessment scales for malingering(self-report scale of brief psychopathological symptoms,SBPS).Conclusion The RSRMDMH has acceptable psychometrics properties on reliability and validity.It is a clinically reliable measure to determine the reliability in mental disorder medical history.